eDNA For Environmental Monitoring

In a New Zealand first, researchers have developed a nationwide database to integrate and share eDNA data to allow biological diversity across our diverse landscapes to be assessed and compared.

This research is Completed

Overview

The analysis of eDNA requires the extraction and identification of DNA directly from environmental samples such as soil or freshwater.

Environmental DNA, or eDNA, refers to the DNA that is shed or excreted from biological organisms, for example as skin, hair, faeces or urine. It provides a window into the world of biological diversity that is otherwise largely hidden from view.

This powerful new technology is transforming how biological diversity is measured. It is being used by a BioHeritage research team led by Dr Gavin Lear, University of Auckland, to develop a nationwide database – or virtual hub – that integrates eDNA data with existing monitoring programmes.

New Zealand needs state-of-the-art molecular tools, such as eDNA metabarcoding, to detect biosecurity incursions and to assess the state of biodiversity and ecosystem function.

Creating a national database for eDNA information allows these data to be kept in consistent format for use by researchers all over the country, maximising opportunities for biodiversity and biosecurity monitoring.

It has the potential to unite currently disparate data generated across different populations and organisations, plus bring together diverse users including citizen scientists, regional councils, the Department of Conservation, iwi and private landowners.

Highlights

Ultimately, the virtual hub provides robust information that can be used to detect biosecurity threats, assess conservation performance and enable data visualisation.

To date, more than 20 research organisations and consortia are feeding into and using the virtual hub and more are being encouraged to do so. The research team is also collaborating with Australia’s Biomes of Australian Soil Environments (BASE), in line with a strategic intent to partner with relevant international groups in high-impact areas.

In addition to the virtual hub, the research team is exploring potential real-world applications for national biodiversity assessment for primary sectors.

This project is helping the BioHeritage Challenge achieve its goal of empowering New Zealanders so they feel inspired to protect our environment.

Looking for more information?

If you’re looking for any outputs (papers, data etc) from this project that you don’t see on this page please visit our data repository.

Resource outputs from this programme

Publication

DNA metabarcoding as a tool for invertebrate community monitoring: a case study comparison with conventional techniques

When conserving native biodiversity, it is particularly important to consider invertebrates, a diverse and functionally important component of biodiversity. However, their inclusion in monitoring and…
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Publication

Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples

Advances in the sequencing of DNA extracted from media such as soil and water offer huge opportunities for biodiversity monitoring and assessment, particularly where the…
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Publication

Opportunities and limitations for DNA metabarcoding in Australasian plant-pathogen biosecurity

Protecting plants from new pathogen incursions requires effective surveillance practices. Environmental DNA (eDNA) metabarcoding shows considerable promise for detecting invasive organisms in terrestrial and aquatic…
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Publication

A comparison of the ability of PLFA and 16S rRNA gene metabarcoding to resolve soil community change and predict ecosystem functions

Soil bacterial community structure has traditionally been measured using phospholipid fatty acid (PLFA) profiling. However, with the development of high-throughput sequencing technologies and metabarcoding techniques,…
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Publication

Optimal extraction methods for the simultaneous analysis of DNA from diverse organisms and sample types

Using environmental DNA (eDNA) to assess the distribution of micro- and macroorganisms is becoming increasingly popular. However, the comparability and reliability of these studies is…
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Publication

Towards robust and repeatable sampling methods in eDNA‐based studies

DNA-based techniques are increasingly used for measuring the biodiversity (species presence, identity, abundance and community composition) of terrestrial and aquatic ecosystems. While there are numerous…
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Publication

A cross-taxa study using environmental DNA/RNA metabarcoding to measure biological impacts of offshore oil and gas drilling and production operations

Standardized ecosystem-based monitoring surveys are critical for providing information on marine ecosystem health. Environmental DNA/RNA (eDNA/eRNA) metabarcoding may facilitate such surveys by quickly and effectively…
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Publication

Using DNA meta barcoding to assess New Zealand’s terrestrial biodiversity

High throughput DNA sequencing technology has enabled entire biological communities to be characterised from DNA derived from pools of organisms, such as bulk-collected invertebrates, or…
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Publication

Metabarcoding monitoring analysis: the pros and cons of using co-extracted environmental DNA and RNA data to assess offshore oil production impacts on benthic communities

Sequencing environmental DNA (eDNA) is increasingly being used as an alternative to traditional morphological-based identification to characterize biological assemblages and monitor anthropogenic impacts in marine…
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Publication

Following Rapoport’s Rule: the geographic range and genome size of bacterial taxa decline at warmer latitudes

We sought to test whether stream bacterial communities conform to Rapoport’s Rule, a pattern commonly observed for plants and animals whereby taxa exhibit decreased latitudinal…
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Publication

Bacteria as Emerging Indicators of Soil Condition

Bacterial communities are important for the health and productivity of soil ecosystems and have great potential as novel indicators of environmental perturbations. To assess how…
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Publication

Aspect has a greater impact on alpine soil bacterial community structure than elevation.

Gradients in environmental conditions, including climate factors and resource availability, occur along mountain inclines, providing a ‘natural laboratory’ to explore their combined impacts on microbial…
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